6 0 non resorbable monofilament prolene suture Search Results


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( A ) Representative FACS histograms of CD3 − CD49b + expanded murine NK cells (i) and expanded NK cells after incubation with <t>FITC-labeled</t> (ii) α-CD16 NPs, (iii) α-4-1BB NPs, (iv) α-EGFR NPs, (v) α-CD16/α-4-1BB NPs, and (vi) α-EGFR/α-CD16/α-4-1BB NPs. ( B ) Representative CLSM images of CD3 − CD49b + expanded murine NK cells after incubation with FITC-labeled (i) α-CD16 NPs, (ii) α-4-1BB NPs, (iii) α-EGFR NPs, (iv) α-CD16/α-4-1BB NPs, and (v) α-EGFR/α-CD16/α-4-1BB NPs. ( C ) Representative FACS histograms of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). a.u., arbitrary unit; MFI, median fluorescence intensity. ( D ) Representative CLSM images of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). ( E ) Direct in vitro toxicities of free EPI, nontargeted EPI NPs, and different antibody-functionalized EPI NPs against (i) HT29, (ii) MB468, and (iii) A431 cells, as assessed by MTS assay 3 days after initial treatment. ( F ) Representative CLSM images of α-γ-H2AX–stained A431 cells after being treated with different EPI formulations for 18 hours.
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( A ) Representative FACS histograms of CD3 − CD49b + expanded murine NK cells (i) and expanded NK cells after incubation with <t>FITC-labeled</t> (ii) α-CD16 NPs, (iii) α-4-1BB NPs, (iv) α-EGFR NPs, (v) α-CD16/α-4-1BB NPs, and (vi) α-EGFR/α-CD16/α-4-1BB NPs. ( B ) Representative CLSM images of CD3 − CD49b + expanded murine NK cells after incubation with FITC-labeled (i) α-CD16 NPs, (ii) α-4-1BB NPs, (iii) α-EGFR NPs, (iv) α-CD16/α-4-1BB NPs, and (v) α-EGFR/α-CD16/α-4-1BB NPs. ( C ) Representative FACS histograms of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). a.u., arbitrary unit; MFI, median fluorescence intensity. ( D ) Representative CLSM images of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). ( E ) Direct in vitro toxicities of free EPI, nontargeted EPI NPs, and different antibody-functionalized EPI NPs against (i) HT29, (ii) MB468, and (iii) A431 cells, as assessed by MTS assay 3 days after initial treatment. ( F ) Representative CLSM images of α-γ-H2AX–stained A431 cells after being treated with different EPI formulations for 18 hours.
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( A ) Representative FACS histograms of CD3 − CD49b + expanded murine NK cells (i) and expanded NK cells after incubation with <t>FITC-labeled</t> (ii) α-CD16 NPs, (iii) α-4-1BB NPs, (iv) α-EGFR NPs, (v) α-CD16/α-4-1BB NPs, and (vi) α-EGFR/α-CD16/α-4-1BB NPs. ( B ) Representative CLSM images of CD3 − CD49b + expanded murine NK cells after incubation with FITC-labeled (i) α-CD16 NPs, (ii) α-4-1BB NPs, (iii) α-EGFR NPs, (iv) α-CD16/α-4-1BB NPs, and (v) α-EGFR/α-CD16/α-4-1BB NPs. ( C ) Representative FACS histograms of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). a.u., arbitrary unit; MFI, median fluorescence intensity. ( D ) Representative CLSM images of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). ( E ) Direct in vitro toxicities of free EPI, nontargeted EPI NPs, and different antibody-functionalized EPI NPs against (i) HT29, (ii) MB468, and (iii) A431 cells, as assessed by MTS assay 3 days after initial treatment. ( F ) Representative CLSM images of α-γ-H2AX–stained A431 cells after being treated with different EPI formulations for 18 hours.
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( A ) Representative FACS histograms of CD3 − CD49b + expanded murine NK cells (i) and expanded NK cells after incubation with <t>FITC-labeled</t> (ii) α-CD16 NPs, (iii) α-4-1BB NPs, (iv) α-EGFR NPs, (v) α-CD16/α-4-1BB NPs, and (vi) α-EGFR/α-CD16/α-4-1BB NPs. ( B ) Representative CLSM images of CD3 − CD49b + expanded murine NK cells after incubation with FITC-labeled (i) α-CD16 NPs, (ii) α-4-1BB NPs, (iii) α-EGFR NPs, (iv) α-CD16/α-4-1BB NPs, and (v) α-EGFR/α-CD16/α-4-1BB NPs. ( C ) Representative FACS histograms of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). a.u., arbitrary unit; MFI, median fluorescence intensity. ( D ) Representative CLSM images of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). ( E ) Direct in vitro toxicities of free EPI, nontargeted EPI NPs, and different antibody-functionalized EPI NPs against (i) HT29, (ii) MB468, and (iii) A431 cells, as assessed by MTS assay 3 days after initial treatment. ( F ) Representative CLSM images of α-γ-H2AX–stained A431 cells after being treated with different EPI formulations for 18 hours.
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( A ) Representative FACS histograms of CD3 − CD49b + expanded murine NK cells (i) and expanded NK cells after incubation with <t>FITC-labeled</t> (ii) α-CD16 NPs, (iii) α-4-1BB NPs, (iv) α-EGFR NPs, (v) α-CD16/α-4-1BB NPs, and (vi) α-EGFR/α-CD16/α-4-1BB NPs. ( B ) Representative CLSM images of CD3 − CD49b + expanded murine NK cells after incubation with FITC-labeled (i) α-CD16 NPs, (ii) α-4-1BB NPs, (iii) α-EGFR NPs, (iv) α-CD16/α-4-1BB NPs, and (v) α-EGFR/α-CD16/α-4-1BB NPs. ( C ) Representative FACS histograms of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). a.u., arbitrary unit; MFI, median fluorescence intensity. ( D ) Representative CLSM images of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). ( E ) Direct in vitro toxicities of free EPI, nontargeted EPI NPs, and different antibody-functionalized EPI NPs against (i) HT29, (ii) MB468, and (iii) A431 cells, as assessed by MTS assay 3 days after initial treatment. ( F ) Representative CLSM images of α-γ-H2AX–stained A431 cells after being treated with different EPI formulations for 18 hours.
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( A ) Representative FACS histograms of CD3 − CD49b + expanded murine NK cells (i) and expanded NK cells after incubation with <t>FITC-labeled</t> (ii) α-CD16 NPs, (iii) α-4-1BB NPs, (iv) α-EGFR NPs, (v) α-CD16/α-4-1BB NPs, and (vi) α-EGFR/α-CD16/α-4-1BB NPs. ( B ) Representative CLSM images of CD3 − CD49b + expanded murine NK cells after incubation with FITC-labeled (i) α-CD16 NPs, (ii) α-4-1BB NPs, (iii) α-EGFR NPs, (iv) α-CD16/α-4-1BB NPs, and (v) α-EGFR/α-CD16/α-4-1BB NPs. ( C ) Representative FACS histograms of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). a.u., arbitrary unit; MFI, median fluorescence intensity. ( D ) Representative CLSM images of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). ( E ) Direct in vitro toxicities of free EPI, nontargeted EPI NPs, and different antibody-functionalized EPI NPs against (i) HT29, (ii) MB468, and (iii) A431 cells, as assessed by MTS assay 3 days after initial treatment. ( F ) Representative CLSM images of α-γ-H2AX–stained A431 cells after being treated with different EPI formulations for 18 hours.
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( A ) Representative FACS histograms of CD3 − CD49b + expanded murine NK cells (i) and expanded NK cells after incubation with <t>FITC-labeled</t> (ii) α-CD16 NPs, (iii) α-4-1BB NPs, (iv) α-EGFR NPs, (v) α-CD16/α-4-1BB NPs, and (vi) α-EGFR/α-CD16/α-4-1BB NPs. ( B ) Representative CLSM images of CD3 − CD49b + expanded murine NK cells after incubation with FITC-labeled (i) α-CD16 NPs, (ii) α-4-1BB NPs, (iii) α-EGFR NPs, (iv) α-CD16/α-4-1BB NPs, and (v) α-EGFR/α-CD16/α-4-1BB NPs. ( C ) Representative FACS histograms of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). a.u., arbitrary unit; MFI, median fluorescence intensity. ( D ) Representative CLSM images of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). ( E ) Direct in vitro toxicities of free EPI, nontargeted EPI NPs, and different antibody-functionalized EPI NPs against (i) HT29, (ii) MB468, and (iii) A431 cells, as assessed by MTS assay 3 days after initial treatment. ( F ) Representative CLSM images of α-γ-H2AX–stained A431 cells after being treated with different EPI formulations for 18 hours.
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( A ) Representative FACS histograms of CD3 − CD49b + expanded murine NK cells (i) and expanded NK cells after incubation with <t>FITC-labeled</t> (ii) α-CD16 NPs, (iii) α-4-1BB NPs, (iv) α-EGFR NPs, (v) α-CD16/α-4-1BB NPs, and (vi) α-EGFR/α-CD16/α-4-1BB NPs. ( B ) Representative CLSM images of CD3 − CD49b + expanded murine NK cells after incubation with FITC-labeled (i) α-CD16 NPs, (ii) α-4-1BB NPs, (iii) α-EGFR NPs, (iv) α-CD16/α-4-1BB NPs, and (v) α-EGFR/α-CD16/α-4-1BB NPs. ( C ) Representative FACS histograms of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). a.u., arbitrary unit; MFI, median fluorescence intensity. ( D ) Representative CLSM images of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). ( E ) Direct in vitro toxicities of free EPI, nontargeted EPI NPs, and different antibody-functionalized EPI NPs against (i) HT29, (ii) MB468, and (iii) A431 cells, as assessed by MTS assay 3 days after initial treatment. ( F ) Representative CLSM images of α-γ-H2AX–stained A431 cells after being treated with different EPI formulations for 18 hours.
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Image Search Results


( A ) Representative FACS histograms of CD3 − CD49b + expanded murine NK cells (i) and expanded NK cells after incubation with FITC-labeled (ii) α-CD16 NPs, (iii) α-4-1BB NPs, (iv) α-EGFR NPs, (v) α-CD16/α-4-1BB NPs, and (vi) α-EGFR/α-CD16/α-4-1BB NPs. ( B ) Representative CLSM images of CD3 − CD49b + expanded murine NK cells after incubation with FITC-labeled (i) α-CD16 NPs, (ii) α-4-1BB NPs, (iii) α-EGFR NPs, (iv) α-CD16/α-4-1BB NPs, and (v) α-EGFR/α-CD16/α-4-1BB NPs. ( C ) Representative FACS histograms of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). a.u., arbitrary unit; MFI, median fluorescence intensity. ( D ) Representative CLSM images of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). ( E ) Direct in vitro toxicities of free EPI, nontargeted EPI NPs, and different antibody-functionalized EPI NPs against (i) HT29, (ii) MB468, and (iii) A431 cells, as assessed by MTS assay 3 days after initial treatment. ( F ) Representative CLSM images of α-γ-H2AX–stained A431 cells after being treated with different EPI formulations for 18 hours.

Journal: Science Advances

Article Title: Trispecific natural killer cell nanoengagers for targeted chemoimmunotherapy

doi: 10.1126/sciadv.aba8564

Figure Lengend Snippet: ( A ) Representative FACS histograms of CD3 − CD49b + expanded murine NK cells (i) and expanded NK cells after incubation with FITC-labeled (ii) α-CD16 NPs, (iii) α-4-1BB NPs, (iv) α-EGFR NPs, (v) α-CD16/α-4-1BB NPs, and (vi) α-EGFR/α-CD16/α-4-1BB NPs. ( B ) Representative CLSM images of CD3 − CD49b + expanded murine NK cells after incubation with FITC-labeled (i) α-CD16 NPs, (ii) α-4-1BB NPs, (iii) α-EGFR NPs, (iv) α-CD16/α-4-1BB NPs, and (v) α-EGFR/α-CD16/α-4-1BB NPs. ( C ) Representative FACS histograms of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). a.u., arbitrary unit; MFI, median fluorescence intensity. ( D ) Representative CLSM images of EGFR-overexpressed HT29, MB468, and A431 cells after incubation with FITC-labeled α-EGFR NPs, α-CD16/α-4-1BB NPs, and α-EGFR/α-CD16/α-4-1BB NPs ( n = 3). ( E ) Direct in vitro toxicities of free EPI, nontargeted EPI NPs, and different antibody-functionalized EPI NPs against (i) HT29, (ii) MB468, and (iii) A431 cells, as assessed by MTS assay 3 days after initial treatment. ( F ) Representative CLSM images of α-γ-H2AX–stained A431 cells after being treated with different EPI formulations for 18 hours.

Article Snippet: Drug-free FITC- and Cy5-labeled NPs were prepared via the same nanoprecipitation method, except that 2.5% w/w of FITC-conjugated poly(lactic acid) (Akina Inc.; catalog number, AV016) or 2.5% w/w of Cy5-conjugated poly(lactic acid) (Akina Inc.; catalog number, AV032) was used instead of EPI.

Techniques: Incubation, Labeling, Fluorescence, In Vitro, MTS Assay, Staining